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Conditions of optimal recombinant Human TRAIL R1/DR4/TNFRSF10A Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP004251: Human TRAIL R1/DR4/TNFRSF10A Protein with the C-hFc Tag
Source: The recombinant Human TRAIL R1/DR4/TNFRSF10A Protein, (amino acids Pro34-Asn239) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human TRAIL R1/DR4/TNFRSF10A protein with the hFc tag at the C-terminus has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human TRAIL protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TRAIL R1 protein with the C-hFc tag with the EC50 of 11.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human TRAIL R1/DR4/TNFRSF10A Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005721: Human TRAIL R1/DR4/TNFRSF10A Protein with the C-His-Avi Tag
Source: The recombinant Human TRAIL R1/DR4/TNFRSF10A Protein, (amino acids Pro34-Asn239) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human TRAIL R1/DR4/TNFRSF10A protein with the His-Avi tag at the C-terminus has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity: The affinity constant of 1.02 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human TRAIL R1/DR4/TNFRSF10A Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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